Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
MOLTOX Molecular Toxicology Inc Saline Solution 0.85% Nacl, 9 mL/tube, 10 tubes/box, Store at Room Temperature
MOLTOX offers a wide array of solutions and buffers for a variety of laboratory research needs. All reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 100G GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade , multicompendial
Gold Biotechnology Inc MES Free Acid 250 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Boston Bioproducts Inc Bis-Tris Buffer (0.2 M, pH 6.5) - 250ml
The Bis-Tris Buffer is a zwitterionic agent commonly used in biological applications. It maintains a pH within 5.8 to 7.2, ideal for biological processes that require stable environments within this range. It is particularly effective in applications such as protein and nucleic acid purification, biochemical assays, and cell culture. The buffering capacity of Bis-Tris is influenced by its concentration, while its pH can be adjusted based on the ratio of Bis-Tris to its conjugate acid or base.
Boca Scientific Inc BuccalFix DNA/RNA Stabilization Buffer, 25ml, Sufficient for Stabilizing 50 swabs
BuccalFix Stabilization Buffer is designed to completely stabilize the DNA and RNA on buccal swabs by inhibiting all enzymatic and microbial activity that occurs following buccal sampling. DNA can be stored in BuccalFix at ambient temperature for over 2 years, while RNA can be stabilized for 4 days. While stabilized, the structure and integrity of the DNA and RNA is fully maintained for further downstream processing applications. Stabilized DNA in BuccalFix can be processed for isolation by the addition of Proteinase K and subsequent isolation steps.
Sigma Aldrich Fine Chemicals Biosciences HEPES Sodium Salt | 75277-39-3 | MFCD00036463 | 100g
HEPES Sodium Salt | Purity: 99% | Mol Wt: 260.29 | 75277-39-3 | MFCD00036463 | 100g
Sigma Aldrich Fine Chemicals Biosciences Trizma acetate | 6850-28-8 | MFCD00038951 | 250g
Trizma acetate | Purity: ≥99% | Mol Wt: 181.19 | 6850-28-8 | MFCD00038951 | 250g
Polysciences, Inc. 1M Glycine-HCl Buffer, pH 2.7±0.1, 10X Concentrate 500 ML
This item has a minimum qty of 2 per supplier requirements.
1M GLYCINE-HCL BUFFER 500 ML
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 100G
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 100G
Growcells TAE BUFFER 50X 1L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
Kd Medical Inc TBE (TRIS-BORATE EDTA), 10X
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 500ml bottle.
Growcells TRIS-EDTA PH8 1L EA
Ready to use, sterile TE (Tris-EDTA) buffer solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. pH 8.0
STEMCELL Technologies HBSS with 10 mM HEPES, Without Phenol Red, Size: 500 mL
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating, or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions, and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
Kd Medical Inc TAE (TRIS-ACETATE EDTA), 50X
TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. 50X concentrated ready to be diluted to a 1X working solution. One 500ml bottle.
Kd Medical Inc TAE 10X 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 10X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.