Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp PIPES FREE ACID ULTRAPURE, 10GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp PIPES FREE ACID ULTRAPURE, 100GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp Phosphate Buffered Saline, 5 L
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
STEMCELL Technologies RoboSep™ Human CD11b Positive Selection and Depletion Kit, For processing: 1 x 10^9 cells
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
Research Products International Corp MES Sodium Salt 5 KG
MES Sodium Salt, also known as 2-(N-Morpholino)ethanesulfonic acid sodium salt, is a zwitterionic buffer commonly used in biological and biochemical research.
In the life science field, MES Sodium Salt is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 5.5 to 6.7, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MES Sodium Salt is commonly used in protein purification, enzyme assays, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MES Sodium Salt is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes.;
Research Products International Corp MES Anhydrous 5 KG
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp BES 500 G
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 4 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 1 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Bioworld TT - SDS Running Buffer Kit (10X), 1 L
TT-SDS Running Buffer 10X KitEach kit contains both a cathode and anode buffer (Catalog #: 765540 and 765542)
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.4, pH Adjusted with KOH) - 500ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.