Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 7.5) - 100ml
PIPES Buffer (0 5 M pH 7 5) - 100ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, particularly in the preparation of enzyme buffers.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Especially useful in the preparation of enzyme buffers
Medchemexpress LLC HEPES hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 g/mol | C8H18N2O4S · 1/2 Na | 10 G
HEPES hemisodium salt is the hemisodium salt form of the zwitterionic buffering agent HEPES, provided at 99.0% purity. It has molecular formula C8H18N2O4S · 1/2 Na and molecular weight 249.80 g/mol. The compound is used to prepare buffers effective near physiological pH for cell culture, enzymatic assays, and general biochemical applications.
- Provides stable buffering in the pH 6.8-8.2 range.
- High purity suitable for biochemical and cell culture applications.
- Readily soluble for convenient buffer preparation.
- Useful for enzymatic assays and general laboratory pH stabilization.
Medchemexpress LLC 1,4-Piperazinedipropanesulfonic acid, β1,4-dihydroxy- | 68189-43-5 | ≥95.0% | 25 G
POPSO is a zwitterionic buffer that increases osmolality and demonstrates significant inhibition of anion uniport. It inhibits chloride uniport with an IC50 value of 24 mM. POPSO also enhances copper uptake and toxicity in alga and impairs the mitochondrial inner membrane. The working pH range for POPSO sesquisodium salt is 7.2-8.5.
- Zwitterionic buffer
- Increases osmolality
- Marked inhibition of anion uniport
- Inhibits chloride uniport with an IC50 value of 24 mM
- Enhances copper uptake and toxicity in alga
- Impairs mitochondrial inner membrane
- Working pH range (for sesquisodium salt): 7.2-8.5
![Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660370.jpg-250.jpg)
Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 100 G
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)) is a biochemical reagent available in a 100g bottle. This buffering agent is commonly used in laboratory settings to maintain a stable pH in aqueous solutions, particularly in biochemistry and molecular biology experiments. HEPPS is frequently used for enzyme assays, protein purification, and cell culture applications where precise pH control is necessary. It has a stable buffering capacity and works well at physiological pH levels, making it ideal for maintaining pH balance in a variety of biological assays. This reagent is essential for accurate experimental results in numerous biochemical processes.
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659772.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 250 G
PIPES (Piperazine N,N' bis(2 ethanesulfonic acid)) is a versatile biochemical reagent used in various applications such as protein studies, electrophoresis, and enzyme assays. This 250g formulation offers a highly stable buffer system with a pH range of 6.8–7.4, ideal for maintaining neutral conditions during biochemical reactions. The 250g quantity is perfect for labs requiring large quantities of buffer for routine experiments or large-scale production. PIPES is widely used in tissue culture, cell biology, and other biochemical research areas that require precise pH control to ensure accurate and reproducible results.
![Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661289.jpg-250.jpg)
Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 500 G
TAPS, or Tris(hydroxymethyl) aminopropanesulfonic acid, is available in a high-purity free acid form for laboratory use. The 500g size is ideal for medium-scale buffer preparation, particularly in research and diagnostics. TAPS is widely used in biochemical, molecular biology, and clinical research applications to maintain a stable pH during experiments. The 500g quantity allows for frequent preparation of buffers without the need for constant restocking, providing researchers with a reliable, long-term supply. TAPS is critical for pH-sensitive reactions, including protein assays, electrophoresis, and enzyme studies, ensuring accuracy and consistency in experimental conditions.
Crescent Chemical Co Inc PROTEIN TEST MIX 5 FOR SDS PG
Protein Test Mixture 5 for SDS PAGE Dalton-marker proteins, low molecular weight, for SDS PAGE Molecular weight markers for SDS gel electrophoresis.Reconstitute with SDS sample buffer (125 mM Tris-HCl pH 6.8, 2 % SDS, 15 % glycerol, 10 mM DTT, 0.025 % bromophenol blue, 0.025 % Orange
Teknova qPCR DNase Buffer. 100mL, Sterile. 10 pack.
10.31mM Magnesium Chloride,10.31mM Calcium Chloride,10.31mM Tris-HCl
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3894476 TRIS USP MULTICOM 50KG FLOWMOR